Informatics Publishing Limited

Madan Swati ¹, Singh Gyanendra Nath ¹, Kumar Yatendra ², Kohli Kanchan ³, Singh Raman Mohan ¹, Om Prakash ¹

Affiliations
1 Central Indian Pharmacopoeia Laboratory, Govt. of India, Ministry of Health and Family Welfare, Sector -23, Raj Nagar, Ghaziabad, U.P.- 201002, IN
2 I.T.S. Paramedical College (Pharmacy), Delhi-Meerut Road, Murad Nagar, Ghaziabad, U.P.- 201206, IN
3 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hamdard University, New Delhi - 110 062, IN

Abstract

Flacourtia indica dichloromethane (DCM), butanol (BuOH), methanol (MeOH) extract of stem bark and methanolic extract of leaves were screened for in vitro antioxidant activity using different models. Subsequent quantification showed the presence of 15.62 and 11.53%w/w phenolics; 1.15 and 1.80%w/ w of flavonol in methanol extract of stem bark and leaf of Flacourtia indica (F. indica) respectively. The methanolic extract showed an effective DPPH radical scavenging activity with low IC₅₀ values of 17.5 and 21 µg/ml respectively and hydroxyl radical scavenging by p-NDA method with IC₅₀ value of 350.23 µg/ml. Butanolic extract showed nitric oxide radical inhibition activity with IC₅₀ value of 28.5 µg/ml. The greater amount of phenolic compounds leads to more potent radical scavenging effect as shown by F. indica extracts. The antioxidant property of the extracts may be due to presence of phenolic content.

Keywords

Flacourtia indica, DPPH, Nitric Oxide Radical Inhibition Assay, Scavenging of Hydroxyl Radical by p-NDA.

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